Journal: Kidney Research and Clinical Practice

Article Title: Pan-Nox inhibitor treatment improves renal function in aging murine diabetic kidneys

doi: 10.23876/j.krcp.23.004

Figure Lengend Snippet: (A) Representative immunoblots for plasminogen activator inhibitor 1 (PAI-1) and type IV collagen (Col4); (B) densitometric analysis of immunoblots for PAI-1 and Col4; (C) representative immunoblot for Klotho, p-FoxO1, t-FoxO1 and Nrf2; and (D) densitometric analysis of immunoblot for Klotho, p-FoxO1/t-FoxO1 ratio, Nrf2. Data are shown as the mean ± standard error of mean. * p < 0.05, p < 0.001 vs. control or APX-115, # p < 0.05, ### p < 0.001 vs. streptozotocin (STZ).

Article Snippet: Proteins were transferred onto a polyvinylidene difluoride membrane, and the membrane was hybridized in blocking buffer overnight at 4 °C with rabbit polyclonal anti-Nox1 antibody (1:1,000; Abcam Plc), rabbit polyclonal anti-Nox2 antibody (1:1,000; Bioworld Technology), rabbit polyclonal anti-Nox4 antibody (1:1,000; Bioworld Technology), rabbit polyclonal anti-PAI-1 antibody (1:1,000; Santa Cruz Biotechnology), mouse monoclonal anti-monocyte chemoattractant protein (MCP)-1 antibody (1:1000; Lsbio), rabbit polyclonal anti-type IV collagen antibody (1:1,000; Abcam Plc), rabbit polyclonal anti-TGF-β1 antibody (1:1,000; Abbkine), goat polyclonal anti-Klotho antibody (1:1,000; R&D Systems Inc.), rabbit polyclonal anti-phospho FoxO1 antibody (1:1,000; Lsbio), rabbit polyclonal anti-total-FoxO1 antibody (1:1,000; Cell Signaling Technology), rabbit polyclonal anti-NRF2 antibody (1:1,000; Cell Signaling Technology), or mouse monoclonal anti-β actin antibody (1:5,000; Sigma-Aldrich).

Techniques: Western Blot, Control

Journal: bioRxiv

Article Title: sKL/mKL Transcript Ratio and Protein Localization Define a Species- and Region-Specific Klotho Signature in the CNS and AD Progression

doi: 10.1101/2025.05.13.653838

Figure Lengend Snippet: Representative images of double immunolabelling sections from human hippocampus and amygdala for total klotho and s-KL (A) ; or for s-KL and GFAP (B) . Negative controls were done by incubating sections without the primary antibodies or by incubating with the pre-adsorbed antibody. White arrows in (B) point to the few neurons expressing s-KL, while yellow labelling indicates s-KL positive astrocytes. Bar scale size is 10μm. Quantification of total soluble klotho (KL1) and s-KL in CSF (C) and homogenized brain tissue (HC: hippocampus, EC: entorhinal cortex) (D) by ELISA.

Article Snippet: The primary antibody, mouse monoclonal klotho antibody F-5 (Santa Cruz Biotechnology, ref sc-515939), diluted 1/100 in blocking buffer, was applied overnight at 4°C.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Animals : an Open Access Journal from MDPI

Article Title: Characterization of Urine-Derived Stromal/Stem Cells from Healthy Dogs and Dogs Affected by Chronic Kidney Disease (CKD)

doi: 10.3390/ani15020242

Figure Lengend Snippet: Klotho protein expression was confirmed by Western blotting. Results are shown as the mean ± SEM. * p < 0.05. Legend: hUSCs = USCs from healthy dogs; sUSCs: = USCs from sick dogs.

Article Snippet: The membrane was cut into two pieces: one was hybridized with 1 μg of mouse monoclonal primary antibody against Klotho protein (sc-515942 Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and the other with 1 μg of anti GAPDH (sc-47724 Santa Cruz Biotechnology, Inc., Dallas, TX, USA) by the SNAP i.d.

Techniques: Expressing, Western Blot

Journal: Cells

Article Title: Asparagine614 Determines the Transport and Function of the Murine Anti-Aging Protein Klotho

doi: 10.3390/cells13201743

Figure Lengend Snippet: List of antibodies used.

Article Snippet: Anti-Klotho , goat, polyclonal , 1:50 in PBSI for immunofluorescence , R&D Systems, AF1819.

Techniques: Western Blot, Immunofluorescence, Blocking Assay

Journal: Cells

Article Title: Asparagine614 Determines the Transport and Function of the Murine Anti-Aging Protein Klotho

doi: 10.3390/cells13201743

Figure Lengend Snippet: List of antibodies used.

Article Snippet: The coverslips were then incubated with anti-Klotho antibody (AF1819, R&D Systems, 1:50 dilution) for 30 min at 4 °C, rinsed 3 times with PBSI, and fixed with ROTI ® Histofix 4% paraformaldehyde on ice for 5 min, followed by room temperature fixation for 20 min.

Techniques: Western Blot, Immunofluorescence, Blocking Assay